Determination of Intracellular Darunavir by Liquid Chromatography Coupled with Fluorescence Detection
نویسندگان
چکیده
منابع مشابه
Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection
Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...
متن کاملDetermination of selenomethionine by high-performance liquid chromatography-fluorescence detection coupled with on-line oxidation.
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of 22 other amino acids within 35 min. Applying an optim...
متن کاملDetermination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection
A sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Posaconazole and internal standard ketoconazole in the methanol extract are subsequently analyzed by...
متن کاملDetermination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection.
This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separa...
متن کاملDetermination of 4-hexylresorcinol in shrimp by liquid chromatography with fluorescence detection.
A method was developed to determine 4-hexylresorcinol in shrimp meat. The procedure is based on extraction of test portions with methanol followed by liquid chromatographic analysis of the extracts, using a reversed-phase column and fluorimetric detection (excitation: 280 nm, and emission: 310 nm). The confidence interval of the recovery in working range of 1.5-2.5 mg/kg was 81.6 +/- 0.8%. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2013
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/bmt147